Search for: All records

Crops rely on microbes for critical services, but host benefits can be influenced by local makeup of microbiota and the host’s capacity to select optimal strains. We investigated host benefits that cowpeas receive from microbiota depending on plant genotype, their domestication status, and soil source.

We performed a full factorial soil inoculation experiment. Twenty diverse cowpea genotypes, selected from wild and domesticated populations, were exposed to soil rinsates from four agricultural sites across California, all having cowpea cultivation and varied physicochemical features. Cowpea investment in and benefit from microbiota was quantified by measuring host growth response to inoculation, nodulation, and segregating trait variation.

Variation in induction of root nodulation and strikingly heterogenous benefits to host growth were observed among soil sites. These effects were restricted to live soil inocula but were absent in autoclaved soil controls that lacked microbiota. Cowpeas expressed heritable variation in nodulation, but there was negligible effect of plant population or domestication status on the net benefit that hosts gained from microbiota.

Soils varied substantially and consistently among cultivation sites and were the most prominent driver shaping host growth effects on cowpeas. While growth benefits vary among host cultivars, soil microbiota (and the conditions that maintain them) predominantly shape plant performance in agricultural settings.

 

Abstract Scattering of high energy particles from nucleons probes their structure, as was done in the experiments that established the non-zero size of the proton using electron beams 1 . The use of charged leptons as scattering probes enables measuring the distribution of electric charges, which is encoded in the vector form factors of the nucleon 2 . Scattering weakly interacting neutrinos gives the opportunity to measure both vector and axial vector form factors of the nucleon, providing an additional, complementary probe of their structure. The nucleon transition axial form factor, F A , can be measured from neutrino scattering from free nucleons, ν μ n  →  μ − p and $${\bar{\nu }}_{\mu }p\to {\mu }^{+}n$$ ν ¯ μ p → μ + n , as a function of the negative four-momentum transfer squared ( Q 2 ). Up to now, F A ( Q 2 ) has been extracted from the bound nucleons in neutrino–deuterium scattering 3–9 , which requires uncertain nuclear corrections 10 . Here we report the first high-statistics measurement, to our knowledge, of the $${\bar{\nu }}_{\mu }\,p\to {\mu }^{+}n$$ ν ¯ μ p → μ + n cross-section from the hydrogen atom, using the plastic scintillator target of the MINERvA 11 experiment, extracting F A from free proton targets and measuring the nucleon axial charge radius, r A , to be 0.73 ± 0.17 fm. The antineutrino–hydrogen scattering presented here can access the axial form factor without the need for nuclear theory corrections, and enables direct comparisons with the increasingly precise lattice quantum chromodynamics computations 12–15 . Finally, the tools developed for this analysis and the result presented are substantial advancements in our capabilities to understand the nucleon structure in the weak sector, and also help the current and future neutrino oscillation experiments 16–20 to better constrain neutrino interaction models.